RNA

Part:BBa_K2787030

Designed by: Weiyi Tang   Group: iGEM18_ShanghaiTech   (2018-10-09)
Revision as of 23:38, 17 October 2018 by Fwhrdfz (Talk | contribs)


pT181 Attenuator target sequence with improved repressive efficiency

An improved version of pT181 sense target with its length shortened from 287bp to 201bp and its repressive efficiency improved from 84%for BBa_ to 98%. The part achieve such efficiency through forming secondary structures that can interact with the pT181 antisense and hinder the transcription of downstream genes. With the absence of pT181 antisense, nevertheless, the expression is basically unaffected.

Usage and Biology

ShanghaiTech2018-pT181-1.png
ShanghaiTech2018-pT181-2.png
ShanghaiTech2018-pT181-3.png
ShanghaiTech2018-pT181-4.png
ShanghaiTech2018-pT181-5.png
ShanghaiTech2018-pT181-6.png
ShanghaiTech2018-pT181-7.png
ShanghaiTech2018-pT181-8.png
ShanghaiTech2018-pT181-9.png
ShanghaiTech2018-pT181-10.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None