Composite
ZntA

Part:BBa_K2832008

Designed by: Michael Taylor and Arianna Sigalos   Group: iGEM18_FSU   (2018-09-09)
Revision as of 23:37, 17 October 2018 by Alexkata15 (Talk | contribs)


ZntR Inducible mRFP1 Generator ( ZntR ---> ZntAp ---> mRFP1 )

Mechanosensitive channels open to mechanical force. Sound being the mechanical force can open the channel. Zinc ions, among others, enters the cell. Zinc cations bind to zntR, zinc-dependent transcription factor. Active zntR binds to zntAp. zntAp upregulates mRFP to show correct induction. Natural systems, zntA upregulates zntA, a zinc export system. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates expression of RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
    Illegal AgeI site found at 765
    Illegal AgeI site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None