Composite

Part:BBa_K2587018

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-17)
Revision as of 23:36, 17 October 2018 by ThomasB (Talk | contribs)


Prpa_RBS_E_T

This construct contains lysis gene E. E is a lysis gene from the bacteriophage PhiX174. This gene is used in our co-culture to induce self lysis of E. coli under the control of the Prpa</i> promoter. This promoter is part of the Quorum sensing system of <i>Rhodopseudomonas palustris. Together with the other components of the system, RpaR and RpaI, this promoter induces expression of the lysis gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93


[edit]
Categories
Parameters
None