Intermediate

Part:BBa_S04038:Design

Designed by: Victoria Hsiao   Group: Guests   (2008-07-28)
Revision as of 03:51, 29 July 2008 by Vhsiao (Talk | contribs) (→‎Source)

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S03977:B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the gene pabB + b0034 RBS placed in front of the b0015 terminator. Eventually this part will be ligated with pabA + the j23100 promoter to create a plasmid containing the two necessary genes of the para-aminobenzoic acid synthesis pathway.

This plasmid can then be overexpressed to test the effects of increasing pABA production on total folate concentrations.

Source

pabB was extracted from the genome of L.lactis subspe. IL1403.

References