Composite
Part:BBa_K2787027
Designed by: Weiyi Tang Group: iGEM18_ShanghaiTech (2018-10-09)
RNA attenuator pT181 with GFP under pT181 target sequence
pT181 is a RNA switch that can repress the expression of target gene by binding its Antisense RNA to its sense target DNA placed upstream of the coding sequenced of target gene. Here the part carries a pT181 Antisense under constitutive promoter J23100 and a GFP reporter whose coding sequence is placed seamlessly after pT181 sense target, also under J23100. This part is used for testing the repressive efficiency of pT181 efficiency by contrast to the results from part BBa_K2787028.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 268
Illegal NheI site found at 291 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1141
[edit]
Categories
Parameters
None |