Intermediate

Part:BBa_S04038:Design

Designed by: Victoria Hsiao   Group: Guests   (2008-07-28)
Revision as of 03:51, 29 July 2008 by Vhsiao (Talk | contribs) (→‎Design Notes)

S03977:B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the gene pabB + b0034 RBS placed in front of the b0015 terminator. Eventually this part will be ligated with pabA + the j23100 promoter to create a plasmid containing the two necessary genes of the para-aminobenzoic acid synthesis pathway.

This plasmid can then be overexpressed to test the effects of increasing pABA production on total folate concentrations.

Source

References