Composite
Part:BBa_K2859002:Experience
Designed by: Kyuhee Jo Group: iGEM18_HAFS (2018-10-10)
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Applications of BBa_K2859002
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UNIQ02eff225f8c6d9f8-partinfo-00000000-QINU UNIQ02eff225f8c6d9f8-partinfo-00000001-QINU
We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.