Composite
Part:BBa_K2859001:Experience
Designed by: Kyuhee Jo Group: iGEM18_HAFS (2018-10-10)
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UNIQbc51c7670c74f4cd-partinfo-00000000-QINU UNIQbc51c7670c74f4cd-partinfo-00000001-QINU
We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.