Composite

Part:BBa_K2859001:Experience

Designed by: Kyuhee Jo   Group: iGEM18_HAFS   (2018-10-10)
Revision as of 23:22, 17 October 2018 by Kyuhee0622 (Talk | contribs) (→‎User Reviews)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2859001

User Reviews

UNIQ517eeadf75921e15-partinfo-00000000-QINU UNIQ517eeadf75921e15-partinfo-00000001-QINU

We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.

SDS.jpg