Coding

Part:BBa_K2686002:Design

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-09-30)
Revision as of 23:11, 17 October 2018 by Caffeine4Life (Talk | contribs) (→‎Design Notes)

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Encapsulin protein with HexaHistidine insert


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457


Design Notes

The HexaHistidine sequence (GGGGGGHHHHHHGGGGG) was introduced in between amino acids 43/44 in BBa_K2686001 which is shifted by one amino acid downstream compared to the location in BBa_K192000