Coding

Part:BBa_K2560128:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-10-08)
Revision as of 23:10, 17 October 2018 by Henselt (Talk | contribs) (→‎Design Notes)


Phytobrick version of K660004


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by polymerase chain reaction (PCR) on the template BBa_K660004 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Forward oligo: AACGTCTCGCTCGAATGATTGAAAAAAACTTTGTGATTACCGATC

Reverse Oligo: TTCGTCTCCCTCAAAGCCACATGATCGCTGCCATCC

Source

As Source DNA we used BBa_BBa_K660004.