Intermediate

Part:BBa_S04035:Design

Designed by: Victoria Hsiao   Group: Guests   (2008-07-28)
Revision as of 21:52, 28 July 2008 by Vhsiao (Talk | contribs) (→‎Source)

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J23100:S03961


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This combines folB (+b0034) + folKE (+b0034) from the folate biosynthesis operon in L.lactis subspe. IL1403 behind the j23100 strong promoter. It exists in the psb1A2 high copy plasmid.

Source

Original genes were extracted from the Lactococcus lactis subspe. IL1403 genome (ordered from ATCC).

References