Intermediate

Part:BBa_S04035:Design

Designed by: Victoria Hsiao   Group: Guests   (2008-07-28)
Revision as of 21:52, 28 July 2008 by Vhsiao (Talk | contribs) (→‎Design Notes)

J23100:S03961


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This combines folB (+b0034) + folKE (+b0034) from the folate biosynthesis operon in L.lactis subspe. IL1403 behind the j23100 strong promoter. It exists in the psb1A2 high copy plasmid.

Source

References