Part:BBa_K2587000
luxI_codon optimized S.cerevisiae
LuxI is an acyl homoserine lactone synthase, most known from the bacterium Aliivibrio fischeri. LuxI is a basic component of the quorum sensing system of A. fischeri. Bacteria use it as a communication module to regulate expression of genes in a cell density-dependent manner. This part contains a variant of this synthase codon optimized for the common yeast, Saccharomyces cerevisiae. Besides the codon optimization, the construct also contains type II S restriction sites (BsaI), useful for example for the Golden Gate assembly cloning method. Since yeast is a eukaryotic organism and the quorum sensing system is encountered in prokaryotes, a codon optimized variant for utilization in an eukaryotic organism represents the beginning of designing quorum sensing-based control systems in eukaryotes. For example, LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter gene or a lysis gene to control cell population.
Usage and Biology
This part can be used as a component for designing synthetic circuits in S.cerevisiae. For instance, in our project, we used this gene to induce expression of a reporter after activation of a synthetic, self designed promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 6
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 689
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 30
Illegal BsaI.rc site found at 673
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