Coding

Part:BBa_K2657003

Designed by: Kim Ly   Group: iGEM18_Austin_UTexas   (2018-10-09)
Revision as of 22:59, 17 October 2018 by MelodyK (Talk | contribs)


Phytobrick of RCP

In the improved BBa_E1010, specific overhangs for MoClo functional elements were added to the flanks of the part to make it into a PhytoBrick. Additionally, BsaI restriction sites were added so that the improved part could be inserted into the Universal Acceptor.

Figure 2. White circles indicates colonies of expected phenotype.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 572
    Illegal AgeI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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