Tag

Part:BBa_K2560060:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-09-18)
Revision as of 22:53, 17 October 2018 by Henselt (Talk | contribs) (→‎Design Notes)


Phytobrick version of 4x BBa_E1010


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 552
    Illegal AgeI site found at 664
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by polymerase chain reaction (PCR) on the template BBa_K2560033 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Forward oligo: AACGTCTCGCTCGAATGGCTTCCTCCGAAGACGTTATC

Reverse Oligo: TTCGTCTCCCTCACATCCCAGCACCGGTGGAGTGACGAC

Source

As Source DNA we used BBa_BBa_K2560033 from our Toolbox.