Composite

Part:BBa_K2787010

Designed by: Haoer Shi   Group: iGEM18_ShanghaiTech   (2018-10-09)
Revision as of 22:45, 17 October 2018 by Fwhrdfz (Talk | contribs)


GFP under B8 orthogonal RBS with orthogonal 16S rRNA A2

The 16S rRNA A2 under constitutive promoter BBa_J23100(BBa_K2787006) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the A2 and B8 ribosome system.


Usage and Biology

ShanghaiTech2018-b8 rbs.png
ShanghaiTech2018-A2 16S.png
A2-B8-C9-Orthogonality.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 798
    Illegal NheI site found at 821
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1660
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707


[edit]
Categories
Parameters
None