Composite

Part:BBa_K2787009

Designed by: Haoer Shi   Group: iGEM18_ShanghaiTech   (2018-10-09)
Revision as of 22:42, 17 October 2018 by Fwhrdfz (Talk | contribs)


GFP under A2 orthogonal RBS with orthogonal 16S rRNA A2

The 16S rRNA A2 under constitutive promoter BBa_J23100(BBa_K2787006) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to justify the proper function of the ribosomal 16SRNA and RBS set A2.

Usage and Biology

ShanghaiTech2018-A2 16S.png
ShanghaiTech2018-A2 A2.png
ShanghaiTech2018-A2 rbs.png
A2-B8-C9-Orthogonality.png
Shanghaitech-A2.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 797
    Illegal NheI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


[edit]
Categories
Parameters
None