Part:BBa_K2859000:Experience

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UNIQd15560b9ab368048-partinfo-00000000-QINU UNIQd15560b9ab368048-partinfo-00000001-QINU

Afther synthesizing the part flanked by biobrick prefix and suffix, we used digested the part and pSB1C3 vector with EcoR1 and Pst1 and ligated the part into the pSB1C3 vector. The resulting plasmid was transformed into E.Coli DH5-α. They were grown overnight at 37°C. The samples were prepared for SDS page gel. The expression of Penetratin-Insulin-Flag tag complex was evaluated with SDS page gel.