Part:BBa_K2533002

RBS-ldhDnARSdR-RBS-lldP

ldhDnARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.

Characterization

This is a two section for lactate produce and transfer part.

Figure1. ldhDnARSdR-lldP

DNA Gel Electrophoretic

At first, we only have two simple genes as ldhDnARSdR and lldP. And then we link them through overlap extension PCR. As the figure shows, we get the genetic stripe about 3000bp which means the overlap extension PCR is successful.

Figure2. Overlap extension PCR of ldhDnARSdR and lldP

Metabolic figure

Figure3. Metabolic figure of cyanobacteria with ldhDnARSdR-lldP

As the figure shows, with the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with ldhDnARSdR-lldP absorb CO₂ into Calvin cycle and produce pyruvate. And then ldhDnARSdR can transfer pyruvate into lactate which will be transported to the environment by lldP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 780
  Illegal BglII site found at 1325
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1461
  Illegal AgeI site found at 1924
  Illegal AgeI site found at 2362
  Illegal AgeI site found at 2521
  Illegal AgeI site found at 2533
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 764
  Illegal BsaI.rc site found at 1035