Part:BBa_K1201000

Derived from serratia marcescens

Chitinase gene; ChiA. Is a processive enzyme which degrades chitin from the reducing end. Suitable for projects which require an enzyme that degrades chitin.

Characterization We add 1 mL 200 m g / L of substrate to a clean test tube, 3mL 0.05 mol / L phosphate buffer , 3 min 50 ° C water bath temperature protection Min, and add 1 mL enzyme solution, mix and heat in 50 degrees water 15 min. After that, we used boiling water to terminate the enzymatic reaction, and add water to a volume of 10 mL. Evenly, the solution was centrifuged at 3000r /min for 10 min. Measure the OD value of the upper clear. Definition of enzyme unit: The amount of enzyme required to produce 1 gram of p-nitroaniline per hour under the above reaction conditions is defined as 1 enzyme activity ( U /m L ).

The calculation formula is: (A-A0)*Enzyme dilution factor/kt. In the formula: A: The absorbed value of the enzymatic hydrolyzate sample after constant volume A is fixed

A0: The absorbance of the post-enzymatic solution blank

T : Enzymatic reaction time, unit: h

k, linear coefficient, 0.0648

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1227
  Illegal NgoMIV site found at 1678
  Illegal AgeI site found at 274
  Illegal AgeI site found at 1381
  
• 1000
  COMPATIBLE WITH RFC[1000]