Coding

Part:BBa_K2725013

Designed by: Eva Steele   Group: iGEM18_Edinburgh_UG   (2018-10-11)
Revision as of 22:18, 17 October 2018 by Evasteele (Talk | contribs)


P1003 Casette with 10 Amber substitutions

P1003 Kanamycin resistance cassette with 10 serine codons in the coding sequence substituted for amber stop codons (TAG): S17Amb, S36Amb, S60Amb, S116Amb, S133Amb, S154Amb, S189Amb, S200Amb, S240Amb, S242Amb. When transformed into E .coli there is no P1003 expression and no kanamycin resistance showing that 10 amber substitutions effectively prevents expression.

T--Edinburgh_UG--400.png 

<img src="http://2018.igem.org/File:T--Edinburgh_UG--prob_per_stop.png" width=“10%" height="10%" align="left">

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None