Coding

Part:BBa_K2619014

Designed by: Haohui Fang   Group: iGEM18_XJTLU-CHINA   (2018-10-07)
Revision as of 21:43, 17 October 2018 by Father (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


nSMase 2 (neutral sphingomyelinase 2 or Smpd3--sphingomyelin phosphodiesterase 3 ) with Kozak sequen

This part is composed of a concensus Kozak sequence with an nSMase2 protein coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1626
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 295
    Illegal BsaI.rc site found at 711
    Illegal BsaI.rc site found at 1617


We conducted experiments to test the exosome production boosting ability of our deigned devices (BBa_K2619014—nSMase and BBa_K2619103—Booster) by co-transfection.

Fig. 1 Exosome production boosting by luciferase assay
Fig. 2 Exosome production boosting by Pierce BCA protein assay
Fig. 3 Exosome production boosting by Nanoparticle tracking assay
Fig. 4 Exosome production boosting by Nanoparticle tracking assay for particles below 100 nm


[edit]
Categories
Parameters
None