Composite

Part:BBa_K2717016:Experience

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)
Revision as of 21:41, 17 October 2018 by ChenyuLiu (Talk | contribs) (→‎Applications of BBa_K2717016)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2717016

The whole pathway can be described in Figure 19.

T--BNU-China--_R19.png

Fig.19 Plasmids constructed for testing TGATG efficiency. This part is the Control group 2.


Considering the mutual influence between two kinds of fluorescence, we design this part to accurately test the efficiency of stop-start codon "TGATG". Flow Cytometer (FCM) was used to measure the bacteria's number and a relative fluorescence. As expectation, both green and pink fluorescence can be detected in some bacteria. Then, we compared the final relative fluorescent intensity with that of TGATG-experimental group （BBa_K2717008）and calculated the efficiency. The specific experiments and data processing can be found in our wiki.

User Reviews

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