Coding

Part:BBa_K2765026

Designed by: Jun Li   Group: iGEM18_BIT-China   (2018-09-30)
Revision as of 21:38, 17 October 2018 by NarrrEn (Talk | contribs)


GAL1p+yno1+CYC1t


This part consists of the promoter GAL1, redox sensor yno1 and the terminator cyc1. The promoter GAL1 is an constitutive promoter from Saccharomyces cerevisiae CEN.PK2-1c-GAL1p-yno1-YNO1t. yno1（yeast NADPH oxidase 1） is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion encodes a genuine NADPH oxidase (NOX enzymes),(see the details in part:BBA-K2765007).And thus the target gene yno1 is induced by galactose and repressed by glucose.as shown in Fig.1.

T--BIT-China--iGEM2018-Partsregulator-Fig.1-geneline.png





Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 669
    Illegal XhoI site found at 2430
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2281


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Categories
Parameters
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