Coding

Part:BBa_K2765026:Design

Designed by: Jun Li   Group: iGEM18_BIT-China   (2018-09-30)
Revision as of 21:23, 17 October 2018 by NarrrEn (Talk | contribs)

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GAL1p+yno1+CYC1t


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 669
    Illegal XhoI site found at 2430
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2281


Design Notes

yno1 is cloned from the genome of CEN.PK2-1C,ligased with pESC plasmid,Pgal1+yno1+cyc1t cloned from pESC plasmid.



Source

S. cerevisiae CEN.PK2-1C


References