Composite

Part:BBa_K2596015:Design

Designed by: Priya Aggarwal, Karthik Ledalla   Group: iGEM18_Stony_Brook   (2018-10-10)
Revision as of 20:36, 17 October 2018 by Kledalla (Talk | contribs) (→‎Source)


RBS + cscB (codon optimized for cyanobacteria)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 498
    Illegal NgoMIV site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made sure that the system would be efficient for cyanobacteria, so we used codon optimization and RBS native to cyanobacteria.


Source

BBa_K2596010 (RBS) from Synechocystis sp. 6803

BBa_K2596005 (cscB) from E. coli

References

LdpA: a component of the circadian clock senses redox state of the cell. Ivleva NB, Bramlett MR, Lindahl PA, Golden SS. EMBO J. 2005 Mar 23;24(6):1202-10. Epub 2005 Mar 10. 10.1038/sj.emboj.7600606 PubMed 15775978