Composite

Part:BBa_K2577007

Designed by: Natalie Farny   Group: iGEM18_WPI_Worcester   (2018-10-10)
Revision as of 20:27, 17 October 2018 by Kylie paige (Talk | contribs)

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AraC regulated ZeAFP

Antifreeze/antimicrobial protein ZeAFP regulated by AraC.


T--WPI_Worcester--COncentrationofProtein.png

This graph shows the concentrations of purified proteins, including ZeAFP, labeled as "Ze" on the graph.

T--WPI_Worcester--CoatedPlates.png
T--WPI_Worcester--MixedPlate.png

These are graphs of biofilm assays that included the protein ZeAFP. The proteins were either mixed into the bacteria or it was used to coat the plate that the biofilm was going to be grown on.

T--WPI_Worcester--Bioflim_Assay_Plate.png

This is a graph of the biofilm production with and without the expression of different antifreeze proteins. An AraC promoter was used to regulate the gene expression for this biofilm assay.

The expression of these proteins was proven on the following gel:

T--WPI_Worcester--ProteinGel1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1367
    Illegal NheI site found at 1417
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1307
    Illegal XhoI site found at 1606
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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