Part:BBa_K2596005

cscB (sucrose permease) from E. coli

This is a sucrose-proton symporter from E. coli.

Usage and Biology

CscB is a cell membrane protein that plays an integral role in transporting sucrose across the membrane as it is a sucrose-proton symporter. It is compatible in many strains of Synechococcus elongatus, including 2973, 6803 and 7942. S. elongatus is unique in that its cell membrane proton gradient is generated by maintaining a surplus of protons inside the cell. Sucrose can then be exported from the cell using the proton gradient.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 481
  Illegal NgoMIV site found at 604
  
• 1000
  COMPATIBLE WITH RFC[1000]

Sucrose Assays

For experimental methods and results on characterization, look at the page for composite part BBa_K2596015.