Composite

Part:BBa_K2656123

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-10-08)
Revision as of 20:22, 17 October 2018 by Carolinarp (Talk | contribs)


pT7-GFPmut3b transcriptional unit

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

TU.
Figure 1. Transcriptional unit assembled. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

  • BBa_K2656000: the BBa_I719005 constitutive strong promoter in its Golden Braid compatible version. Promoter T7 leads into a high protein expression.
  • BBa_K2656010: the B0032 low ribosome biding site in its Golden Braid compatible version
  • BBa_K2656022: the BBa_K2656022 GFPmut3b sequence in its Golden Braid standardized version
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None