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Part:BBa_K2847000:Design

Designed by: Slavil Peykov   Group: iGEM18_Bulgaria   (2018-10-09)
Revision as of 20:21, 17 October 2018 by Slavil (Talk | contribs) (→‎Design Notes)

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Cer sequence from plasmid ColE1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Standard design


If you want to have this part synthesized by IDT, order the following sequence: 

gRNA cloning cassette
gaattcgcggccgcttctagagCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGA
TTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATCGCGCAGTTTTTAGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCC
TCGTAGCCATCGAGATAAACCGCAGAAATCGTGTCAGCCAGCAGCCTGGATTTTTTCGTtactagtagcggccgctgcag


PCR amplify it using the following primers:

C_BioBrick_Prefix-F: TATGAATTCGCGGCCGCTTCTAG

C_BioBrick_Suffix-R: TATCTGCAGCGGCCGCTACTAGTA


and then clone it into a pSB vector of choice.

Source

This part was obtained as gBlock fragment by IDT

References