Composite
Part:BBa_K2717012:Design
Designed by: Chenyu Liu Group: iGEM18_BNU-China (2018-10-09)
RBS2000-GFP-hairpin-GDH-v5 tag-his tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2336
Illegal AgeI site found at 3228 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3210
Illegal BsaI.rc site found at 678
Design Notes
rbs2000-GFP-hairpin-gdh-tag
Source
The glucose dehydrogenase is isolated from the genome of the laboratory strain. V5 tag and His tag are obtained from our own lab. The sequence of rbs2000 is obtained from the Kit and we synthesize by ourselves. The hairpin structure is synthesize by ourselves.