Composite

Part:BBa_K2717012

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)
Revision as of 20:14, 17 October 2018 by ChenyuLiu (Talk | contribs)

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RBS2000-GFP-hairpin-GDH-v5 tag-his tag

In this composite part, we link GFP and glucose dehydrogenase with hairpin structure and use Promoter lacI and RBS 2000 control the whole coding. The hairpin structure contains a 6x His tag, a stop codon and RBS, which make the transcription ratio of the downstream gene and the upstream gene is about 1:0.2. We designed this part to verify the positive feedback regulation mechanism of GDH.

function:

We designed this part to verify the positive feedback regulation mechanism of GDH: when gdh is expressed, the strain gains a growth advantage. At the same time, due to the hairpin structure, the expression level of GFP has been very high. gdh promotes the metabolism of strains, which means it will express more gdh to gain growth advantage, resulting in more GFP being expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2336
    Illegal AgeI site found at 3228
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3210
    Illegal BsaI.rc site found at 678


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Categories
Parameters
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