Regulatory

Part:BBa_K2596001:Design

Designed by: Priya Aggarwal, Jennifer Rakhimov   Group: iGEM18_Stony_Brook   (2018-10-09)
Revision as of 20:06, 17 October 2018 by Kledalla (Talk | contribs) (→‎Design Notes)

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PcpcB from Synechococcus elongatus PCC7942


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made sure to include both cpc promoters.

Source

The origin of the part is the NCBI Synechococcus elongatus PCC7942

References

Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. Application of bioluminescence to the study of circadian rhythms in cyanobacteria. 2000;305:527-42. PubMed 10812624

Masahiko I, et al. (2003). Thymine at —5 Is Crucial for cpc Promoter Activity of Synechocystis sp. Strain PCC 6714. Journal of Bacteriology.

Sawaki H, Sugiyama T, Omata T. Promoters of the Phycocyanin Gene Clusters of the Cyanobacterium Synechococcus sp. Strain PCC 7942. Plant Cell Physiol. 1998;39(7): 756-761.

Zhou J, Zhang H, Meng H, et al. Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Scientific Reports. 2014;4(4500): 1-6. doi: 10.1038/srep04500.