Coding

Part:BBa_K1336002

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-06)
Revision as of 19:46, 17 October 2018 by JimLi2002 (Talk | contribs)


Spore coat protein, laccase, from Bacillus subtilis

CotA gene (1542bp) coding for an enzyme from Bacillus subtilis that is used to degrade and decolourise azo dyes, through an oxidative process.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 867
    Illegal XbaI site found at 1482
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 867
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 867
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 867
    Illegal XbaI site found at 1482
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 867
    Illegal XbaI site found at 1482
    Illegal AgeI site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1509
    Illegal SapI.rc site found at 286


[edit]
Categories
Parameters
None