Part:BBa_K2839008

Insulated Psp1w1 promoter (B0030 RBS)

1. Short Description

This composite part was submitted by iGEM Thessaloniki and consists of Psp1w1 promoter driving the expression of sfGFP fluorescent marker. Psp1w1 promoter has a single operator located between the -35 and -10 elements, where TAL Effector 1 can tightly bind, decoupling its expression from plasmid/gene copy number. Copy number positively affects GOI expression, while its repressor, being under the same positive influence, compensates for this change resulting in the disassociation of the GOI’s expression from the plasmid’s copy number.

Psp1w1 (B0030) device leads to a weaker sfGFP expression when compared to Psp1w1 B0032 device (BBa_2839007) due to the different RBS. RiboJ insulates the translational unit from surrounding sequences. This part is included in our Part Collection, a genetic toolbox for controlled gene expression. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 712
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]