Composite

Part:BBa_K2717005:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-09-22)
Revision as of 19:21, 17 October 2018 by ChenyuLiu (Talk | contribs) (→‎Source)

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mprA-GAPGH promoter-emrr binding promoter-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 76
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2037
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 687


Design Notes

In the course of the experiment, the genes of GAPDH promoter, emrR, emrR binding promoter and mcherry were amplified from the corresponding template by PCR. GAPDH promoter was used to initiate the expression of emrR, and emrR binding promoter is used to start. The expression of the mcherry gene; in the process of constructing the vector, the GAPDH promoter, emrR, emrR binding promoter and mcherry genes are first ligated by the overlap method, and the vector of pUC19 is linearized by the enzyme linearization method through infusion. The GAPDH promoter and emrR gene fragments are ligated, and then the vector was linearly digested, and the emrR binding promoter and mcherry genes were ligated by infusion.

Source

cherry is produced from the plasmid, other genes are from the E. coli’s genome.

References