Coding

Part:BBa_K592001

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-21)
Revision as of 19:13, 17 October 2018 by Kaku (Talk | contribs)

CcaS, green light sensor from Synechocystis sp. PCC6803

CcaS is a green light-induced sensor protein from the genome of Synechocystis sp. PCC6803. It requires PCB as the chromophore to function. PCB is made by ho1 and PcyA. Furthermore, CcaS works in conjunction with CcaR, its response regulator. This is the green light sensor used by Tabor in his multichromatic light-sensing system which composed of CcaS/CcaR and Cph8/EnvZ.

A new part (BBa_K2627000) constructed by SDU-China was improved from CcaS by deleting about 800 bases corresponding to two PAS domains since we were inspired by Professor Koji’s work. Part BBa_K2627000 (CcaS#4) is a varient of CcaS having an opposite response to green/red light. High intensity of fluorescence can be seen when bacteria are cultured under red light while fluorescence is pretty weak under green light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None