Composite

Part:BBa_K2717015

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)
Revision as of 19:01, 17 October 2018 by ChenyuLiu (Talk | contribs)


GFP-TGATG-T7 RNA Polymerase-GDH-v5 tag-his tag

The vector has GFP-TGATG-T7 RNA Polymerase , T7 promoter and gdh, which can increase the growth rate of the strain by positive feedback and delay the senescence of the strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5252
    Illegal AgeI site found at 6144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6126
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
None