Composite

Part:BBa_K2557008

Designed by: Sheng XU   Group: iGEM18_NAU-CHINA   (2018-10-09)
Revision as of 18:50, 17 October 2018 by Brunoinigem (Talk | contribs) (→‎Usage and Biology)

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TP901 attB-RFP-TP901 attP

We added TP901 recombination sites, attB and attP, at both ends of the reporter gene mCherry (Inverted). When PhiC31 is expressed, attB and attP are recognized, and the sequence of mCherry is inverted to enable normal expression.


Sequence and Features

Characterization

Fig. 1 Fluorescence microscope observation of HEK 293T undergone different experimental treatments. Transfection of different amounts of plasmids containing recombinase genes into cells.

Fig. 1 shows that this composite part works fine in the HEK 293T.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 194
    Illegal SapI site found at 811


[edit]
Categories
//chassis/eukaryote
Parameters
None