Coding

Part:BBa_K2717008:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)
Revision as of 18:44, 17 October 2018 by ChenyuLiu (Talk | contribs) (→‎Design Notes)

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GFP-TGATG-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1425
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

We adjusted the codon of the last lysine of GFP from AAA to AAG.

Source

GFP and mCherry is stored in our own lab.

References