Part:BBa_K2557028
TetO-miniCMV promoter- EGFP
The miniCMV promoter and the EGFP coding sequence were ligated in sequence for characterizing the promoter strength.
Sequence and Features
Usage and Biology
This composite part can transduce the input signal of the upstream TEV protease into the fluorescent signal of Enhanced GFP. Here, we characterized the combination of the TetO-miniCMV promoter.
Characterization
Fig.1 Inhibition of tetR on promoter with tetO sequence (A)Fluorescence microscope observation of HEK 293T only transfect with plasmids containing promoters with tetO sequence. (B)Fluorescence microscope observation of HEK 293T transfect with plasmids containing promoters with tetO sequence and tetR.
Fig. 1A shows that the strength of the three promoters in HEK 293T cells is from strong to weak: Ubc > EF1 α > miniCMV. Fig. 1B shows that TetR has obvious inhibitory effects on the three promoters downstream of TetO.
References
1. Ramos, J. L. et al. The TetR Family of Transcriptional Repressors The TetR Family of Transcriptional Repressors. Microbiol. Mol. Biol. Rev. 69, 326–356 (2005).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 325
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 331
- 1000COMPATIBLE WITH RFC[1000]
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