Reporter

Part:BBa_K2557037

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)
Revision as of 18:07, 17 October 2018 by Brunoinigem (Talk | contribs)

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EGFP

Enhanced Green Fluorescent Protein is a fluorescent protein and is a widely used reporter gene in synthetic biology.


Characterization

Fig. 1 Fluorescence microscope observation of HEK 293T only transfect with plasmids containing promoters with tetO sequence.

Fig. 1 shows that EGFP can be normally expressed in HEK 293T, and the fluorescence intensity depends on the amount of expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 6
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote
Parameters
None