Composite
jqy

Part:BBa_K2717017:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)
Revision as of 18:05, 17 October 2018 by ChenyuLiu (Talk | contribs) (→‎Source)

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mprA-protection coding3-mCherry reverse-emrr binding promoter reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 776
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 556


Design Notes

NOTHING

Source

Sequences were attained from E. coli F12K using PCR. After adding lacO and rbs in the 5’ of mpra while v5 and 6*his tag in the 3’ of it, we fused the altered sequence with emrr Binding Promoter and mCherry.

References