Part:BBa_K2888000
cyanophage lysozyme gene
This cyanophage lysozyme gene can target to and specifically lyse the cell wall of cyanobacteria by cooperating with Bugbuster chemical solution. Among the 27 lysozyme gene family, this cyanophage lysozyme gene gives the most efficient and effective soluble expression as well as purification. Unlike other bacteria, cyanobacteria have much thicker layers which are difficult to be lysed with common enzymes. Therefore, cyanobacteria-targeted lysozyme and other chemicals breaking through these unique barriers are needed in chemical lysis process.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Application
We intend to use lysozyme gene to lyse cyanoabacteria which has unusually thick and complex cell wall. The cyanobacterium has a much thicker peptidoglycan layer and a unique external layer containing an S-layer and exopolysaccharide. In our experiment, we easily mix Bugbuster and expressed protein in order to fully lyse cyanobacteria cell wall
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