Composite

Part:BBa_K2717013:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)
Revision as of 17:57, 17 October 2018 by ChenyuLiu (Talk | contribs) (→‎Source)


TorA signal-VHb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 540
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

VHb


Source

We obtained the sequence expressing the VHb protein from part BBa_K1321200 by PCR, and obtained the TorA signal sequence by gene synthesis. We linked these two sequences to the linearized vector by infusion. We placed the TorA sequence upstream of the VHb sequence and initiated expression by a promoter.

References