Coding

Part:BBa_K2570021:Design

Designed by: Luying He   Group: iGEM18_FJNU-China   (2018-10-05)
Revision as of 17:57, 17 October 2018 by Who care (Talk | contribs) (→‎Design Notes)


dsrA+B0034+GFP+B0032+mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 721


Design Notes

dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.

We obtained the wild-type mazf toxin protein sequence from E. coli and conducted experiments as a positive control.

Source

It comes from E.coli.


References

1] Repoila, F. and S. Gottesman (2003). "Temperature Sensing by the dsrA Promoter." Journal of Bacteriology 185(22): 6609-6614.