Coding

Part:BBa_K2587008

Designed by: Susanne Vollmer   Group: iGEM18_Duesseldorf   (2018-09-18)
Revision as of 17:15, 17 October 2018 by Kai H (Talk | contribs)


guaD

guaD is coding for an enzyme, called Guanin Deaminase. It converts guanin to xanthin and ammonia. It can be used as the second step in the degradation of melamin to ammonia, as shown by Shaw et al in 2016. It converts ammeline to ammelide.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
    Illegal NotI site found at 2
    Illegal NotI site found at 1378
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
    Illegal AgeI site found at 840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 25
    Illegal BsaI.rc site found at 1364


References

Shaw, A. Joe, et al. "Metabolic engineering of microbial competitive advantage for industrial fermentation processes." Science 353.6299 (2016): 583-586.
http://science.sciencemag.org/content/353/6299/583?casa_token=_q3c5eJ3Gv4AAAAA:3HmgrxE_r8XSnR8YUtF3cLZ9I-luClCJEJcwxy6E_J711AgFcrN7i-ozHCnV_HZQmxteX12dXxw5rMc

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Categories
Parameters
None