Coding

Part:BBa_K2587005

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-15)
Revision as of 17:10, 17 October 2018 by Kai H (Talk | contribs)


lasR

LasR is the regulator of the quorum sensing system from Pseudomonas aeruginosa. In this case the sequence is codon optimised for E. coli and together with LasI and Plas</i> is supposed to induce expression of a gene of interest in a population density dependent manner.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731

[edit]
Categories
Parameters
None