Part:BBa_K2826008
copper sensor1
A device to detect the copper ion
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 898
Illegal AgeI site found at 1010 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The device contains a copperA promoter,a riboJ,a RBS,a RFP repoter and two terminator. This device will be used to detect copper in solution. The copA promoter is active in the presence of copper. RiboJ can reliably maintain relative promoter strengths. RiboJ helps reduce the leakage of copA promoter greatly. After adding copper ions, the expression of red-fluorescent protein increased steadily.
CopA, the principal copper efflux ATPase in Escherichia coli, is induced by elevated copper in the medium.The copA promoter is Copper-responsive and regulated by CueR,a Member of the MerR Family.
Copper ion detection experiment
experimental method：
2.5 ml of LB solution was added with 2.5 ml of sterilized ultrapure water, and BBa_K2826013 broth was added to make a final concentration of 0.02 OD,cultured at 37 degrees on a shaker for 10 hours. 100 microliters of bacterial solution was taken every 2 hours to detect red fluorescence. When measuring wastewater, replace 2.5 ml of ultrapure water with 2.5 ml of waste water.
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