Part:BBa_K2557000
Anti-GFP-mnotch-TEV protease-NLS
The Notch receptor is a natural cell surface receptor whose extramembrane domain can be replaced by a variety of receptors, and the intramembrane domain can be replaced with a variety of transcription factors to construct a customized cell signaling pathway. We retained the conserved mmotch transmembrane domain, replacing the extracellular domain with anti-GFP and the intracellular domain with the TEV protease, thereby converting the extracellular signal into an intracellular signal.
Usage and Biology
Cells receive extracellular signals and respond to the signal molecules accordingly. We hope to customize a kind of receptor so that it can recognize the signal molecules and regulate downstream gene expression [1]. We choose synNotch as an ideal receptor. Anti-GFP means Lag16.
Characterization
Verification of the localization of Lag16-synNotch-TEV on cell membrane
Fig. 1 (Left) Fluorescence microscope observation of the cells cross-linked with GFP. (Right) Blank control (without transfection).
Fig. 1 shows that synNotch can be located to the membrane. Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 409
Illegal PstI site found at 453
Illegal PstI site found at 772 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 16
Illegal PstI site found at 409
Illegal PstI site found at 453
Illegal PstI site found at 772
Illegal NotI site found at 1570 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2063
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 409
Illegal PstI site found at 453
Illegal PstI site found at 772 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 409
Illegal PstI site found at 453
Illegal PstI site found at 772
Illegal NgoMIV site found at 208
Illegal NgoMIV site found at 913
Illegal NgoMIV site found at 1090
Illegal NgoMIV site found at 1628
Illegal AgeI site found at 25 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1249
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